Department Chair
M. Scott Goodman, Ph.D.
Date of Award
5-2014
Access Control
Campus-Only Access
Degree Name
Forensic Science, M.S.
Department
Chemistry Department
Advisor
Dr. Joonyeong Kim
Department Home page
http://chemistry.buffalostate.edu/forensic-science-ms-4
First Reader
Joonyeong Kim, Ph.D., Associate Professor of the Chemistry
Second Reader
M. Scott Goodman, Ph.D., Chair and Associate Professor of the Chemistry
Third Reader
Jinseok Heo, Ph.D., Assistant Professor of Chemistry
Abstract
Quantitative analysis of trans-10-hydroxy-2-decenoic acid (10-HDA) and adenosine in pure form and dietary supplements of royal jelly available in the United States was carried out via reversed phase high performance liquid chromatography (RP-HPLC). The target compounds, 10-HDA and adenosine, in samples and their internal standards, methyl 4-hydroxybenzoate (MHB) and theophylline, were separated using a Zorbex Eclipse XDB-C18 column (150 × 4.6 mm) with a polar mobile phase. In the case of 10-HDA analysis, a mixture of methanol, water, and phosphoric acid (55:45:5, v/v/v) was used as a mobile phase. The flow rate was 1.0 mL/min and the UV detection was performed at 215 nm at 25 °C. Our results show that the concentration of 10-HDA lies between 1.85 and 2.18 wt% for fresh royal jelly creams and between 0.43 and 6.28 wt% for royal jelly supplements. Adenosine was extracted by sonication in a mixture consisting of ethanol and deionized water (5:95, v/v) and a mobile phase of deionized water and acetonitrile (93:7, v/v) at 25 °C. The flow rate of a mobile phase was set to 1.0 mL/min and the UV detection was performed at 260 nm. The concentration of adenosine lies between ~27 and ~50 mg/g for fresh royal jelly creams and between ~2 and ~173 mg/g for royal jelly supplements.
Recommended Citation
Hagerty, Sheila A., "Quantification of trans-10-Hydroxy-2-Decenoic Acid and Adenosine in Royal Jelly Products Purchased in USA via HPLC" (2014). Forensic Science Theses. 4.
https://digitalcommons.buffalostate.edu/forensic_science_theses/4
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