Department Chair

M. Scott Goodman, Ph.D., Professor of Chemistry

Date of Award


Access Control

Open Access

Degree Name

Forensic Science, M.S.


Chemistry Department


Camila Sabatini, D.D.S, M.S.

Department Home page

First Reader

Camila Sabatini, D.D.S, M.S.

Second Reader

Jooneyang Kim, Ph.D.

Third Reader

M. Scott Goodman, Ph.D.


The purpose of this study was to investigate the biocompatibility and micromechanical properties of newly synthesized antibacterial monomer and cross-linker functional surfactants into a commercially available dental adhesive (Single Bond, 3M ESPE, Saint Paul, MN, USA) at three concentrations (0.05, 0.1, 0.25 mg/mg). All groups were analyzed by evaluation of micro-tensile bond strength, ultimate tensile strength, cell viability, antibacterial properties, and surface micro-hardness. Scanning electron microscopy (SEM) was used for interfacial characterization.

Human extracted molars were used as a substrate for bonding adhesives for the micro-tensile bond strength (MTBS) and scanning electron microscopy (SEM) studies. Twenty resin-dentin beams (0.9 ± 0.1 mm2) per group were evaluated at 24 hrs and 6 mos for MTBS. Slabs of ~1 mm were analyzed in the SEM for surface characterization. For the ultimate tensile strength (UTS), ten hour-glass shaped specimens (10 × 2 × 1mm) per group were tested at 24 hrs, 1 wk, and 6 mos. To evaluate toxicity, four disc-shaped specimens (5 × 2 mm) per group were incubated with human gingival fibroblasts (HGF). Antibacterial properties were evaluated by incubating three disc-shaped specimens (8 × 1 mm) per group with a strain of caries-producing bacteria S. mutans.

Within the limitations of this in vitro study, it was concluded that incorporation of antibacterial monomer and cross-linker additives may be a viable option to help increase the longevity of tooth-colored adhesive restorations. Single Bond adhesive modified with 0.1 mg/mg monomer appears to provide the optimal balance for biocompatibility and micromechanical properties.