M. Scott Goodman, Ph.D.
Date of Award
Forensic Science, M.S.
Dr. Joonyeong Kim
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Joonyeong Kim, Ph.D., Associate Professor of the Chemistry
M. Scott Goodman, Ph.D., Chair and Associate Professor of the Chemistry
Jinseok Heo, Ph.D., Assistant Professor of Chemistry
Quantitative analysis of trans-10-hydroxy-2-decenoic acid (10-HDA) and adenosine in pure form and dietary supplements of royal jelly available in the United States was carried out via reversed phase high performance liquid chromatography (RP-HPLC). The target compounds, 10-HDA and adenosine, in samples and their internal standards, methyl 4-hydroxybenzoate (MHB) and theophylline, were separated using a Zorbex Eclipse XDB-C18 column (150 × 4.6 mm) with a polar mobile phase. In the case of 10-HDA analysis, a mixture of methanol, water, and phosphoric acid (55:45:5, v/v/v) was used as a mobile phase. The flow rate was 1.0 mL/min and the UV detection was performed at 215 nm at 25 °C. Our results show that the concentration of 10-HDA lies between 1.85 and 2.18 wt% for fresh royal jelly creams and between 0.43 and 6.28 wt% for royal jelly supplements. Adenosine was extracted by sonication in a mixture consisting of ethanol and deionized water (5:95, v/v) and a mobile phase of deionized water and acetonitrile (93:7, v/v) at 25 °C. The flow rate of a mobile phase was set to 1.0 mL/min and the UV detection was performed at 260 nm. The concentration of adenosine lies between ~27 and ~50 mg/g for fresh royal jelly creams and between ~2 and ~173 mg/g for royal jelly supplements.
Hagerty, Sheila A., "Quantification of trans-10-Hydroxy-2-Decenoic Acid and Adenosine in Royal Jelly Products Purchased in USA via HPLC" (2014). Forensic Science Theses. Paper 4.