Department Chair

M. Scott Goodman

Date of Award

5-2024

Access Control

Open Access

Degree Name

Forensic Science, M.S.

Department

Chemistry Department

Advisor

Dr. Joonyeong Kim

First Reader

Dr. Joonyeong Kim

Second Reader

Dr. Jinseok Heo

Third Reader

Dr. Sujit Suwal

Abstract

Hemp and marijuana consumption in the United States can be dated back many centuries as being an essential component of the medicinal, industrial, and recreational markets while maintaining its extremely controversial status amongst the scientific and political communities. While researchers suggest that the plant in which both hemp and marijuana are derived from, Cannabis Satvia, contains more than 600 chemicals, the vast majority of research has been conducted on cannabinoids, which account for about 60 of them. Major phytochemicals from cannabis include its psychoactive agent, tetrahydrocannabinol (THC), and non-psychoactive cannabidiol (CBD), cannabigerol (CBG), and many others. Employing the use of high-performance liquid chromatography (HPLC) and additional techniques, these substances within supplements can be separated, identified, and quantified.

The goals of this experiment include quantifying the amount of CBD and CBG in three commercially available hemp supplements while identifying the best extraction solvent and time and testing the thermal stability of the cannabinoids within the supplements. Cannabinoids, CBD and CBG, were successfully identified using HPLC as well as the best extraction solvent and time. The thermal stability of the hemp infused supplements was also identified using HPLC while the identities of the cannabinoids and any unknowns were analyzed by gas chromatography-mass spectrometry (GC-MS) in an attempt to confirm or deny their presence in the supplements.

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